Education

• 1973 Ph.D. Biology (Biophysical Cytology Graduate Group; Shinya Inoue, Thesis Advisor). The University of Pennsylvania, Philadelphia, Pennsylvania.
• 1967 I.A.E.S.T.E. Exchange student to the University of Hannover, Hannover, Federal Republic of Germany.
• 1966 B.S., Applied Biology, The Georgia Institute of Technology, Atlanta, Georgia.
  

Research Interests

• Kinetics of NFkB activation and nuclear translocation in myocardial and vascular endothelial cells.
• The role of cytokines, ROS and NO in ischemia-reperfusion induced inflammation injury of the vascular endothelium and heart.
  

Professional Experience

• 2004- Research Associate Professor, Department of Cell, Developmental Biology and Anatomy, University of South Carolina School of Medicine. Columbia, South Carolina.
  
• 1995-2004 Research Assistant Professor of Medicine, Center of Excellence for Arthritis and Rheumatology, Louisiana State University Medical Center School of Medicine in Shreveport, Shreveport, Louisiana
  
• 2000. Director of Laser Scanning Con-Focal Microscopy Core Facility.
  
• 1989-1994 Director of Electron Microscopy, Research Assistant Professor of Anatomy, Louisiana State University Medical Center School of Medicine in Shreveport, Department of Cellular Biology and Anatomy, Shreveport, Louisiana.
  
• 1985-1988. Associate Professor, Department of Biology, University of Southwestern Louisiana, Lafayette, Louisiana.
  
• 1983-1985. (1) Assistant Professor, Department of Biology, Tulane University, New Orleans, Louisiana. (2) Adjunct Assistant Professor of Anatomy, Department of Anatomy, Tulane University Medical School, New Orleans, Louisiana.
  
• 1983. Participant in the Marine Biology Laboratory Course: Protein Analysis by Polyacrylamide Gel Electrophoresis (Presented by Dr's. Ray Stephens and Alferd Zweidle).
  
• 1980-1983. Research Assistant Professor, Department of Cell Biology, The University of Texas Health Science Center at Dallas, Dallas, Texas.
  
• 1978-1980. Assistant Instructor, Department of Cell Biology, The University of Texas Health Science Center at Dallas, Dallas, Texas.
  
• 1975-1977. Postdoctoral Fellowship and Instructor, Department of Human Biological Chemistry and Genetics, Division of Cell Biology, The University of Texas Medical Branch, Galveston, Texas (Bill R. Brinkley, Sponsor).
  
• 1973-1975. Postdoctoral Fellowship and Research Investigator ship, Marine Biological Laboratory, Woods Hole, Massachusetts (Shinya Inoue, Supervisor).

AWARDS and SUPPORT

• 1995-present. LSU Center of Excellence for Arthritis and Rheumatology.
  
• 2000. Millennium Pharmaceuticals, Inc. Effects of IkB kinase inhibitors in experimental arthritis. Co-Principal Investigator with MB Grisham (P.I.): $37,404.
  
• 1996-2001. N.I.H. Program Project Grant. Pathophysiology of intestinal ischemia- reperfusion. Assistant Director of the Morphology Core: $4,400,000.
  
• 1996-1997. Proscript Corporation. Effect of the 26S proteasome inhibitors on pro inflammatory cytokine expression in PG/PS-induced chronic joint inflammation in LEW/N rats. Principle investigator: $8,000.
  
• 1995-1996. Biomedical Research Foundation ( BRF ) Support Grant. A model of murine arthritis induced by bacterial PG/PS.Principal Investigator: $4,890.
  
• 1992. Louisiana Educational Quality Support Fund. Laser Scan Confocal Microscope.
  Co-Principal Investigator: $356,956.
  
• 1991-1992. N.I.H. Biomedical Research Support Grant. Response of calcium metabolism and microtubule function in fetal cardiac cells exposed to ethanol. Principal Investigator: $7,372.
  
• 1991-1996. N.I.H. Program Project Grant. Pathophysiology of intestinal ischemia- reperfusion. Assistant Director of the Morphology Core: $4,700,000.
  
• 1989-1990. N.I.H. Biomedical Research Support Grant. Teratogenic effect of alcohol on cardiac myofibril development. Principal Investigator: $6,454.
  
• 1989-1990. R. Clifton Brooks Biomedical Fellowship. Effect of hypoxic and anoxic conditions on lysosomes and myofibrillar contractility in cardiac myocytes. Principal Investigator: $12,000.
  
• 1987. Louisiana Educational Quality Support Fund. Cryoprotection of proteins.
  Co-Principal Investigator: $148,000.
  
• 1986. Louisiana Educational Quality Trust Fund Grant. A plan to stimulate competitive research in biology. Additional Investigator: $499,724.
  
• 1985. National Science Foundation. Equipment grant to purchase a transmission electron microscope. Co-Principal Investigator: $150,000.
  
• 1984-1985. American Heart Association -- Louisiana Affiliate. Reversible cardiac lysosomal hypertrophy in mammalian myocytes. Principal Investigator: $9,076.
  
• 1984-1985. American Cancer Society. The morphological and biochemical alteration of intermediate filaments in epithelial cells during induced neoplastic progression. Principal Investigator: $7,500.
  
• 1983. N.I.H. Biomedical Research Support Grant. The response of cardiac lysosomes to lysosmotropic aminoacid methyl esters and hypoxic conditions in cultured mammalian cardiac myocytes. Principal Investigator: $6,307.
  
• 1981-1982. University of Texas Health Science Center at Dallas Biomedical Research Support Grant. Role of intermediate filaments in cardiac myofibrillar assembly. Principal Investigator: $4,000.
  
• 1980-1981. American Cancer Society. Morphological changes of cytoplasmic intermediate (10nm) filaments associated with the transformation of cells in culture.
  Principal Investigator: $4,500.
  
• 1980-1982. American Heart Association. 10nm filaments in myocardial cell development.
  Principal Investigator: $44,000.
  
• 1979-1980. Muscular Dystrophy Association. The structure and function of tubulin in Muscular Dystrophy. Co-Principal Investigator: $27,000.
  
• 1979-1980. American Heart Association -- Texas Affiliate. Intermediate filaments in myocardial cell development. Principal Investigator: $14,023.
  
• 1977-1978. American Cancer Society. The University of Texas Health Science Center at Dallas, Cancer Center Grant in Aid of Research. Morphological changes in cytoplasmic fibrous proteins associated with the transformation of epithelial cells in culture. Principal Investigator: $4,500.
  
• 1968-1973. H.E.W. Predoctoral Traineeship.
  
• 1967. International Association for Exchange Students for Technical Experience
  (I.A.E.S.T.E.) Exchange Student Fellowship, 1 year in Germany.
  

Publications

1. Fuseler, J.W. 1973: Repetitive procurement of mature gametes from individual sea stars and
sea urchins. J.Cell Biol., 57, 879-880.
2. Fuseler, J.W. 1975: Mitosis in Tilia americana. J. Cell Biol., 64, 159-171.
3. Fuseler, J.W. 1975: The dependence of anaphase chromosome
velocity and microtubule depolymerization on temperature. J. Cell Biol., 67, 789-800.
4. Inoue, S., Fuseler, J.W., Salmon, E.D., and G.W. Ellis 1975: Functional organization of mitotic microtubules -- physical chemistry of the in-vivo equilibrium system. Biophys J., 15, 725-744.
5. Miller, C. L., Fuseler, J.W., and B. R. Brinkley 1977: Cytoplasmic microtubules in transformed mouse X nontransformed human epithelial cell hybrids: Correlation with in-vitro growth. Cell, 12, 319-331.
6. Brinkley, B. R., Miller, C. L., Fuseler, J.W., Pepper, D. A., L. J. Wible 1977: The cytoskeleton and cell transformation to malignancy. The Cancer Bulletin, 29, 13-17.
7. Brinkley, B. R., Miller, C. L., Fuseler, J.W., Pepper, D. A., L. J. Wible 1977: Cytoskeletal changes in cell transformation to malignancy. In: Cell Differentiation and Neoplasia (G.F. Saunders, ed.), Raven Press, N.Y. 419-450.
8. Shay, J.W., Peters, T.T., and J.W. Fuseler 1978: Cytoplasmic transfer of microtubule organizing centers in mouse culture cells. Cell, 14, 835-845.
9. Fuseler, J.W., Miller, C. L., and G.W. Fuller 1978: Alterations of cytoskeletal morphologies and growth patterns in human fibroblast treated with polyethylene glycol. Cytobiologie, 18, 345-359.
10. Shay, J.W. and J.W. Fuseler 1979: Diminished microtubules in cells derived from inherited dystrophic muscle explants. Nature, 278, 178-180.
11. Kleve, M.G., Fuseler, J.W., and W.H. Clark 1979: Antibodies against invertebrate actin: Their phytogenic cross reactivity. J. Expt. Zool., 209, 21-31.
12. Shay, J.W., Cook, J., Fuseler, J.W., Feit, H., Thomas, L.E., and J.L. Studt 1979: Microtubules and Muscular Dystrophy. Trans. of the Amer. Neurol. Assoc., 104, 12-15.
13. Shay, J.W., Thomas, L.E., and J.W. Fuseler 1980: Altered mononuclear cell spreading and microtubules in Duchenne Muscular Dystrophy patients and carriers. In: Diseases of the Motor Unit: (D. L. Scotland, ed.), John Wiley and Sons, Inc. 789-801.
14. Fuseler, J.W., Shay, J.W., and H. Feit 1981: The role of intermediate (10nm) filaments in the development and integration of the myofibrillar contractile apparatus in the embryonic mammalian heart. In: Muscle and Cell Motility: (R.M. Dowben and J.W. Shay eds.), vol. 1. Plenum Press, N.Y. 205-257.
15. Fuseler, J.W., Eckert, B.S. and J.W. Shay 1982: The association of creatinephosphokinase with the mitotic spindle. In: Muscle and Cell Motility: (R.M. Dowben and J.W. Shay eds.), vol. 11. Plenum Press, N. Y. 103-119.
16. Shay, J.W., Fuseler, J.W., Neueck, U., Lorkowski, G., Stauver, M. and H. Feit 1982: Cytoskeletal defects in avain muscular dystrophy. In: Muscle and Cell Motility: (R.M. Dowben and J.W. Shay eds.), vol. 11. Plenum Press, N. Y. 121-140.
17. Bollon, A. P., Porterfield, R. R., Fuseler, J.W. and J.W. Shay 1982: Microtubules and heart cell contraction. In: Muscle and Cell Motility: (R.M. Dowben and J.W. Shay eds.), vol. 11. Plenum Press, N. Y. 93-10.
18. Fuseler, J.W. and J.W. Shay 1982: Association of desmin with developing myofibrils of cultured embryonic rat heart myocytes. Development Biol., 91, 448-457.
19. Feit, H., Cook, J. D., and J.W. Fuseler 1983: Myofibrillar creatinkinase in Duchenne and avian muscular dystrophy. Biochem. Med., 29, 355-359.
20. Buchanan, G.R., Smith, S.J., Holtkamp, C.A. and J.W. Fuseler 1983: Bacterial infection and splenic reticuloendothelial function in children with hemoglobin SC disease. Pediatrics, 72, 93-98.
21. Decker, R. S. and J.W. Fuseler 1984: Methylated amino acids and lysosomal function in cultured heart cells. Exp. Cell Res., 154, 302-309.
22. Decker, R. S., Decker, M. L., Thomas, V. and J.W. Fuseler 1985: Response of culture cardiac myocytes to lysosomotropic compounds and methylated amino acids. J. Cell Sci., 74, 119-135.
23. Inoue, S., Bajer, A., Fuseler, J. W., E. D. Salmon 1990: Experiments on the mitotic spindle viewed in polarized light. In: Cellular Motile Processes: Molecules and Mechanisms, Cell Motility and the Cytoskeleton Video, Supplement 2: Microtubule Based Motility. Editor: J. Sanger. Wiley-Liss, NY.
24. Weidner, E., Overstreet, R.M., Tedeschi, B., and J. W. Fuseler 1990: Cytokeratin and desmoplactin analogues within an intracellular parasite. Biol. Bull., 179, 287-294.
25. Lew, D. and J.W. Fuseler 1991: The effect of stepwise expansion on the mitotic activity and vascularity of subdermal tissue and induced capsule. J. Oral and Maxillofacial Surg. 49, 848-853.
26. Korthuis, R.J., Carden, D.L., Shepro, D., and J. W. Fuseler. 1991: Phalloidin and antimonide attenuate ischemia / reperfusion induced neutrophil infiltration and increased microvascular permeability. J. Applied Physiol., 71, 1261-1269.
27. Lew, D. and J.W. Fuseler 1993: The effect of pulsed expansion of subfascially placed expanders on the extent and duration of capsular mitosis in the capsule and rat integument. J. Oral and Maxillofacial Surg., 51, 154-158.
28. Fuseler, J.W. 1993: Maternal alcohol consumption induces transient compensatory hyperplasia of developing cardiac tissue in the neonatal rat. Alcohol and Alcoholism, 28, 657-666. 29. Welbourne, T.C. and J.W. Fuseler 1993: Growth hormone-enhanced acid production and glutamate and glutamine utilization in LLCPK-F+ cells. Am. J. Physiol, 265, (Endocrinol. Metab. 28): E874-E879.
30. Payne, D.K., Fuseler, J.W. and M.W. Owens 1994: Modulation of endothelial cell permeability by lung carcinoma cells: A potential mechanism of malignant pleural effusion formation. Inflammation, 18, 407-417.
31. Jerrells TR., Slukvin I., Sibley DA., Fuseler JW. 1994. Increased susceptibility of experimental animals to infectious organisms as a consequence of ethanol consumption. Alcohol Alcoholism. Suppl 2: 425-430.
32. Sibley, D.A., Fuseler, J.W., Slukvin, I., and T.R. Jerrells 1995: Ethanol-induced depletion of lymphocytes from the mesenteric lymph nodes of C57Bl/6 mice is associated with RNA but not DNA degradation. Alcohol Clin.Exp.Res. 19, 324-333.
33. Fuseler, J.W., Conner, E.M., Davis, J.M., Wolf, R.E., Grisham, MB. 1997. Cytokine and nitric oxide production in the acute phase of bacterial cell wall-induced arthritis. Inflammation. 21: 119-137.
34. Aiko, S., Conner E.M., Fuseler J. W., Grisham M. B. 1997. Effects of cyclosporin or FK506 in chronic colitis. J. Pharmacol. Exp. Ther. 280: 1075-1084.
35. Conner E.M., Brand S., Davis J.D., Laroux F.S., Palombella V.J., Fuseler J.W., Kang D., Wolf R. E., Grisham M. B. 1997. Proteasome inhibition attenuates nitric oxide synthase expression, VCAM-1 transcription and the development of chronic colitis. J. Pharmacol. Exp. Ther. 282: 1615-1622.
36. Morise, Z., Komatsu, S., Fuseler, J. W., Granger, D. N., Perry M., Issekutz, A. C., Grisham M. B.1998. ICAM-1 and P-selectin expression in a model of NSAID-induced gastropathy. Am J Physiol 274. (Gastrointestinal. Liver Physiol. 37): G246-G252.
37.Akio, S. Fuseler, J. W., Grisham M. B. 1998. Effects of nitric oxide synthase inhibition or sulfasalazine on the spontaneous colitis observed in HLA-B27 transgenic rats. J Phram and Exptl Therapeutics. 284: 1-6.
38. Taylor, A. E., Khimenko, P. L., Babgy, G. J., Fuseler, J. W. 1998. Tumor necrosis factor ischemia and reperfusion injury in rat lungs. J Applied Physol. 85: 2005-2011.
39. Palombella, V. J., Conner, E.M, Fuseler, J.W., Destree, A., Davis, J.M., Laroux, F. S., Wolf, R. E., Huang, J., Brand, S., Elliot P. J., Lazarus, D., McCormack, T., Parent, l., Stein, S., Adams, J., Grisham, M. B. 1998. Role of the proteasome and NF-kB in streptococcal cell wall-induced polyarthritis. Proc. Natl. Acad. Sci. 95: 15671-15676.
40. Morise, Z., Granger, D.N., Fuseler, J.W., Anderson D.C., Grisham, M. B. 1999 indomethacin induces gastropathy in CD18, intercellular adhesion molecule 1, or P-selectin deficient mice. Gut. 45: 523-528.
41. Kalogeris, T., Gray, L., Laroux F. S., Cockrell, A., Fuseler, J.W., Conner E.M., Brand, S., and Grisham , M.B. 1999. Selective proteasome inhibitors as anti-inflammatory agents. Exp. Opin. Invest. Drugs. 8(9): 1397-1407.
42. Fuseler. J. W. Hearth-Holmes,M, Grisham, M. B., Kang, D., Laroux, F.S., Wolf, R. E. 2000. FK506 attenuates developing and established joint inflammation and suppresses interleukin-6 and nitric oxide expression in bacterial cell wall-induced polyarthritis. J Rheumatology. 27: 190-199.
43. Kawachi, S., Morise, Z., Conner, E., Laroux, F. S., Gray L., Fuseler, J. W., Grisham, M. B. 2000. E-Selectin expression in a murine model of chronic colitis. Biochem. Biophys. Res. Comm. 268: 547-552.
44. Iwakiri, R., Gotoh, Y., Sugihara, H., Fujimoto, K., Fuseler, J. W., Aw T.Y. 2001. Programmed cell death in rat intestine: effect of feeding and fasting. Scandinavian J. Gastroenterology. 36: 39-47.
45. van der Hyde, H.C., Bauer, P., Sun, G., Chang, W-L., Yin, L., Fuseler, J.W., Granger, D.N. 2001. Assessing vascular permeability during experimental cerebral malaria by a radiolabeled monoclonal antibody technique. Infection and Immunity. 69: 3460-3465.
46. Alexander, J.S., Zhu, Y., Elrod, J.W., Alexander, B., Coe, L., Kalogeris, T.J., Fuseler, J.W. 2001. Reciprocal regulation of endothelial substrate adhesion and barrier function. Microcirculation. 8: 389-401.
47. Tedesco, L. S., Fuseler, J.W., Grisham, M., Wolf, R., Roerig, S.C. 2001. Therapeutic administration of nitric oxide synthase inhibitors reverses hyperalgesia but not inflammation in a rat model of polyarthritis. Pain 1: 215-223.
48. Stokes,K.Y., Clanton,E. C., Bowles, K. S., Fuseler, J. W., Chervenak, D., Chervenak, R., Jennings,S.R., Granger, D.N. 2002. The role of T-lymphocytes in hypercholesterolemia-induced leukocyte-endothelial interactions. Microcirculation. 9: 407-417.
49. Pavlick, K.P., Laroux, F.S., Fuseler, J.W., Wolf, R.E., Gray, L., Hoffman, J., Grisham, M.B. 2002. Role of reactive metabolites of oxygen and nitrogen in inflammatory bowel disease. Free Rad Biol. Medicine. 33: 311-322.
50.Tsunada, S., Iwakiri, R., Noda, T., Fujimoto, K., Fuseler, J.W., and Aw, T. Y., (2002). Chronic exposure to subtoxic levels of peroxidized lipids suppresses mucosal cell turnover in rat small intestine and the reversal by glutathione. Scand. J. Gastroenterology. 36: 39-47.
51. Mailhes, J.B., Hilliard, C., Fuseler, J. W., London S.N. 2003. Vanadate, an inhibitor of tyrosine phosphatases, induced premature anaphase in oocytes and aneuploidy and polyploidy in mouse bone marrow cells. Mutation Res. 538: 101-107.
52. Pias E.K., Ekshyyan O.Y., Rhoades, C.A., Fuseler, J W., Harrison L., Aw, T.Y. 2003. Differential effects of Superoxide dismutase isoform expression on hydroperoxide-induced apoptosis in PC-12 cells. J. Biol. Chem. 278: 13294-13301.
53. Tsunada S., Iwakiri, R., Noda, T., Fujimoto, K., Fuseler, J., Rhoads, C. A., Aw, T.Y. 2003. Chronic exposure to subtoxic levels of peroxidized lipids suppresses mucosal cell turnover in rat small intestine and reversal by glutathione. Digestive Dis. and Sci. 48: 210-222.
54. Mailhes, J.B., Hilliard, C., Fuseler, J.W., London, S.N. 2003. Okadaic acid, an Inhibitor of protein phosphatase 1 and 2A, induces premature separation of sister chromatids during meiosis I and aneuploidy in mouse oocytes in vitro. Chrom. Res. 11: 619-631.
55. Kimpel, D., Grisham, M., Fuseler, J., Gray, L., Kannan, K., Dayton, C., Wolf, R., 2003. Splenectomy attenuates streptococcal cell wall-induced arthritis and alters leukocyte activation. Arthritis and Rheum. 48: 77-89.
56. Laroux F.S., Norris H.H., Houghton J., Pavlick K.P., Bharwani S., Merrill D.M., Fuseler J.W., Chervenak R., Grisham M.B. 2004. Regulation of chronic colitis in athymic nu/nu (nude) mice. International Immunol. 16: 77-89.
57. Mailhes, J.B., Mastromatteo, C., Fuseler, J.W. 2004. Transient exposure to the Eg5 kinesin inhibitor Monastrol leads to syntelic orientation of chromosomes and aneuploidy in mouse oocytes. Mutation Res. 559: 153-167.
58. Fuseler. J.W., F. Laroux, F.S., Merrill ,D.M., Grisham, M.B., Wolf, R.E. 2005. Kinetics of NF-kB Nuclear Translocation in Endothelial Cells. Cell Motl and Cytoskeleton. (In Revision).
59. Fuseler, J.W., Laroux, F.S., Merrill, D.M., Tedesto, L., Roerig, S.C., Grisham, M.B., Wolf, R.E. 2005. Selective Inhibition of Inducible Nitric Oxide Synthase (iNOS) Exacerbates Established Chronic Joint Inflammation and Increases Interleukin-6 Expression in Bacterial Cell Wall-Induced Polyarthritis. J Rheumatology. (In Revision).
60. Rogers J.A. and Fuseler J.W. 2005. The inhibition or activation of nuclear translocation of nuclear factor-kappa B by nitric oxide in human umbilical endothelial cells is dependent on the state of activation of the target cells. (in preparation).
61. Fuseler J.W., Carver W, Jani P, and Millette C.F. 2005. Activation and subcellular Localization of the Rho family Small GTPase, Cdc-42, in response to mechanical stretch in neonatal cardiac fibroblasts (in preparation).