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Title:
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PUBMED Link:
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Research:
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The general research interests of the lab are the mechanisms of embryology. More
specifically, how a single genetic blueprint, created at fertilization, governs the
development of animals. Using the model of the developing heart we are investigating the
molecular mechanisms that regulate growth, differentiation, and ultimately organogenesis.
Both in vivo and in vitro experimental models are used to investigate these developmental
mechanisms. In collaboration with Dr. Mike Yost’s laboratory (USC Dept. of
Surgery), we have been involved in the production and development of a novel
three-dimensional, tubular culturing system. The tube shape of the engineered type I
collagen scaffold mimics the early developing heart tube and serves as an in vivo-like
context on which to study the development of cardiac tissues (Evans et al, 2003). A number
of cell types and combinations of cell types (tissues) have been grown on these engineered
collagen tubes. One use of this model system has been to investigate mechanisms that
regulate the cell cycle of cardiac myocytes. We have focused on the role of a specific
protein, Cenp-f, in the differentiation and proliferation of cardiac myocytes. This
protein participates in the regulation of the cardiac cell cycle by interacting with
members of Rb family of tumor suppressors and the structural/mechanical aspects of the
mitotic spindle. By interacting with regulators of the cell cycle and the mitotic spindle,
Cenp-f participates in the molecular mechanisms of cell division during the development of
shape or morphogenesis (Evans et al, Submitted). Another use of the tubular culturing
system has provided the opportunity to investigate molecular regulatory circuits at higher
levels of biological organization such as the tissue/tissue interactions that are critical
in organogenesis. In collaboration with the lab of Dr. Jay Potts, this has been used to
model the later stages of cardiac valve development (Goodwin et al, 2005). We are
currently investigating the interplay of signaling proteins and physical forces that
regulate cardiac valve formation. An investigation of coronary vascular development has
recently been undertaken in the laboratory and has generated some promising results. These
studies will not only contribute to the understanding of developmental processes, but also
provide useful insights into the tissue engineering field. Thus, by studying development,
other important biomedical goals such as replacement therapies and tissue regeneration
will be forwarded. |
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Recent Publications:
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- Nesbitt, T, A Lemley, J Davis, M J Yost, RL Goodwin, Jay D. Potts. Epicardial
Development in the Rat: A New Perspective. Microscopy and Microanalysis. (In Press).
- Potts, JD, MJ Yost, RL Goodwin 2006. Models of Cardiovascular Development: New
Approaches are Making In Vitro En Vogue. Curr Card Reviews. (2) 55-63.
- Dees, E, JB Robertson, M Ashe, LM Pabón-Peña, D Bader, and RL Goodwin 2005. LEK1
Protein Expression in Normal and Dysregulated Cardiomyocyte Mitosis. Anat Rec A Discov Mol
Cell Evol Biol Sep;286(1):823-32.
- Goodwin, RL, T Nesbitt, RL Price, JC Wells, and JD Potts. 2004. A Three-Dimensional
Model of Valvulogenesis. Devl. Dynamics (In Press).
- Evans, HJ, JK Sweet, RL Price, M Yost, RL Goodwin. 2003. A novel 3-D culture system for
the study of cardiac myocyte development. Am J. Phys Heart and Circulation Physiology
Aug;285(2):H570-8 .
- Yost MJ, CF Baicu, CE Stonerock, RL Goodwin, RL Price, JM Davis, H Evans, P D Watson, CM
Gore, J Sweet, L Creech, MR Zile and L Terracio. A Novel Tubular Scaffold for
Cardiovascular Tissue Engineering. Tissue Eng 10:273-284.
- Narasimham, SV, RL Goodwin, TK Borg, DM Dawson, and BZ Gao. (2004). Multiple Beam Laser
Cell Micropatterning System. SPIE Proceedings 5514: 437-445,
- HJ Evans, L Edwards, RL Goodwin. 2005.Analyses of Murine Cenp-f Reveals a Conserved
Function as a Cell Cycle Checkpoint Protein. (In Review).
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Education:
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- 1985 BS Biological Sciences Indiana University, Bloomington, IN
- 1996 PhD Biology University of South Carolina, Columbia, SC
- 1996-2000 Research Fellow Division of Cardiology, Vanderbilt University School of
Medicine, Nashville, TN (Dr. DM Bader, Mentor)
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Contact Information:
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Email: rgoodwin@med.sc.edu
Phone: 803-733-3313
Address: Building1 Room B-17
USC School of Medicine
Columbia, SC 29209
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| Columbia, SC 29208 • 803-777-7000 • info@sc.edu |
©
2002 University of South Carolina Board of Trustees |
Cell and Developmental
Biology and Anatomy
Columbia, SC 29208
(803) 733-3369 --- (803) 733-1533 fax
Email Us at dba@gw.med.sc.edu |
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