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Holly A. LaVoie, Ph.D.
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Title:
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Associate Professor |
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PUBMED Link:
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LaVoie
HA or La Voie HA |
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Research:
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Our lab studies the hormone-mediated transcription of genes controlling
steroidogenesis in the ovary. One of our main interests had been in the Steroidogenic
acute regulatory (StAR) protein gene. StAR mediates the rate-limiting step of cholesterol
delivery to the mitochondrion for steroid synthesis. Our previous work cloned the LDL
receptor and StAR gene promoters in the pig and showed that both were responsive to the
hormones FSH and LH in granulosa cells. The gonadotropin effects on these promoters were
augmented by IGF-I. Further analysis of the StAR promoter identified GATA4 and C/EBPbeta
as co-mediators of the FSH/IGF-I effect. We found that FSH stimulated the phosphorylation
of GATA4 on a PKA site. These previous studies have led to our current work with GATA4.
Our goal is to identify the true GATA4 responsive gene pool(s) during follicular and
luteal development. Along these lines we are studying the role of phosphorylation in GATA4
transactivation. StAR expression is suppressed in the granulosa layer during follicular
development. One of interests is to determine how StAR remains quiescent during this
period. These studies have led us to investigate histone modifications within the StAR
gene that may keep it silent before the midcycle gonadotropin surge that causes ovulation
and terminal differentiation of the follicular cells into luteal cells. |
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Recent Publications:
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- LaVoie, H.A. Epigenetic control of ovarian function: the emerging role of histone
modifications. Mol. Cell. Endocrinol. 243:12-18, 2005 (review).
- Gillio-Meina, C., Hui, Y.Y., and LaVoie, H.A. Expression of CCAAT/enhancer binding
proteins alpha and beta in the porcine ovary and regulation in primary granulosa cell
cultures. 72:1194-1204, Biol Reprod, 2005.
- Rusovici, R., Hui, Y.Y., and LaVoie, H.A. EGF-mediated inhibition of FSH-stimulated StAR
gene expression in porcine granulosa cells is associated with reduced histone H3
acetylation. Biol Reprod, 72:862-871, 2005.
- LaVoie, H.A., McCoy, G.L., and Blake, C.A. Expression of GATA-4 and GATA-6 in the rat
fetal gonad and the ovary during development and pregnancy. Mol Cell Endocrinol 227:31-40,
2004.
- LaVoie, H.A., Singh, D., and Hui, Y.Y. Concerted regulation of the porcine StAR gene
promoter activity by FSH and IGF-I in granulosa cells involves GATA-4 and C/EBPbeta.
Endocrinology 145:3122-3134, 2004.
- LaVoie, H.A. The role of GATA in mammalian reproduction. Exp Biol Med 228:1282-1290,
2003 (review).
- Rusovici, R. and LaVoie, H.A. Expression and distribution of AP-1 transcription factors
in the porcine ovary. Biol Reprod 69:64-74, 2003.
- Gillio-Meina, C., Hui, Y.Y. and LaVoie, H.A. GATA-4 and GATA-6 transcription factors:
expression, immunohistochemical localization, and possible function in the porcine ovary.
Biol Reprod 68:412-422, 2003.
- LaVoie, H.A., DeSimone, D.C., Gillio-Meina, C. and Hui, Y.Y. Cloning and
characterization of porcine ovarian estrogen receptor beta isoforms. Biol Reprod 66(3),
66:616-623, 2002.
- Sekar, N., LaVoie, H.A. and Veldhuis, J.D. Concerted regulation of steroidogenic acute
regulatory gene expression by luteinizing hormone and insulin (or insulin-like growth
factor I) in primary cultures of porcine granulosa-luteal cells. Endocrinology 141:
3983-3992, 2000.
- LaVoie, H.A., Garmey, J.C., and Veldhuis, J.D. Mechanisms of insulin-like growth factor
I augmentation of follicle-stimulating hormone-induced porcine StAR gene promoter activity
in granulosa cells. Endocrinology 140: 146-153, 1999.
- LaVoie, H.A., Garmey J.C., Day, R.N., and Veldhuis, J.D. Concerted regulation of low
density lipoprotein receptor gene expression by follicle-stimulating hormone and
insulin-like growth factor I in porcine granulosa cells: promoter activation, mRNA
stability, and sterol inhibition. Endocrinology 140: 178-186, 1999 .
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Education:
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- College of William and Mary
B.S., Biology 1987
Minor Anthropology
Williamsburg, Virginia
- College of William and Mary
M.A., with thesis
Biology 1989
Williamsburg, Virginia
- Medical College of Virginia
Ph.D., Physiology, March 1994
Virginia Commonwealth University
Richmond, Virginia
- University of Virginia
Postdoctoral Training 1994-97
Dept. of Internal Medicine
Molecular and Reproductive
Endocrine Division
Endocrinology
- Additional training:
-Introduction to Molecular and Cellular Research Course,
Endocrine Society 1/1994
-cDNA Library Techniques, LT Training Center 6/1994
-Frontiers in Reproduction Course, Marine Biological Laboratories 5-6/2000
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Contact Information:
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hlavoie@med.sc.edu
phone: 803-733-1575 |
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| Columbia, SC 29208 803-777-7000 info@sc.edu |
©
University of South Carolina Board of Trustees |
Cell and Developmental
Biology and Anatomy
Columbia, SC 29208
(803) 733-3159 --- (803) 733-3153 fax
Email Us at dba@gw.med.sc.edu |
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