ANAT 700 - Transmission & Scanning Electron Microscopy ( Fall Semester )

• The course is lab oriented and will emphasize hands on use of the equipment. Routine methods for specimen preparation and image acquisition for scanning and transmission electron microscopy will be covered.
  
• Students will be expected to develop a project in which they will prepare their specimens and present a written report complete with scanning and transmission electron micrographs. Grades will be based on student effort during the course and the quality of the micrographs obtained.

ANAT 740 - Optical Methods ( Spring Semester )

General Information about the Course...

Click here for 2007 syllabus

• The course will be lab oriented and will emphasize hands on use of equipment. Each topic will be introduced by 2-3 hours of lecture followed by several hours of lab work. A computer background in Windows will be helpful, but not essential. Grades will be based on completion and quality of assigned tasks on each piece of equipment. Students will be encouraged to use their own research samples to complete assigned tasks.
  
• A $250 fee will be charged to cover supplies and use of equipment needed to complete the course.
  
• Course enrollment is limited to 8 per semester. Approval of the IRF Director is required prior to enrollment.

  LIGHT MICROSCOPY

  Preparation of sections from paraffin embedded and frozen samples
  Routine and specialized staining techniques
  In situ hybridization techniques
  Digital Photography through the light microscope

  DIGITAL IMAGING, IMAGE ANALYSIS AND IMAGE ENHANCEMENT

  Applications of digital imaging
  Digital Imaging Techniques in microscopy
  Importation of gels and other images for computer analysis, automated object counts and area determinations, image enhancement techniques

  CONFOCAL MICROSCOPY

  Theory and use of the BioRad MRC 1024 confocal scanning laser microscope (CSLM)
  Preparation of fluorescently labelled samples for CSLM
  Amira 3D reconstruction of images generated on BioRad CSLM

  CELL SORTER (FACILITY FOR THE FLUORESCENT ANALYSIS OF CELLS AND TISSUES)

  Theory and use of the Beckman-Coulter Epics XL Flow Cytometer
  Techniques will include calcium and antigen level determination in cells

  PHOSPHORIMAGER

  Theory & use of the BioRad FX Phosphorimager
  Analysis of gene arrays
  Quantitative analysis of protein expression

  SCANNING AND TRANSMISSION ELECTRON MICROSCOPY

  An introduction and overview of scanning and transmission EM as correlative techniques for the above methods. Detailed EM methods will be presented during the fall semester EM course.