Electron Microscopy Systems

JEOL 200CX Transmission Electron Microscope

Description- The JEOL 200CX transmission electron microscope is a standard TEM system for imaging biological ultrastructure. The IRF system is equipped with:

• Tungsten filament
• Resolution of 0.2nm
• Side entry eucentric goniometer stage with +/-30 tilt
• Specimen exchange rod with slots for two 3mm grids
• Accelerating voltages of 80,100,120,160 and 200 kV
• Low and high magnification operating modes
• Film cassette for up to 50 sheets of 3.25 X 4 inch film
• Advanced Microscopy Techniques side mounted 2K digital camera

Applications-

• High resolution ultrastructural imaging
• Immunocytochemistry with collodial gold and other electron dense probes
• Stereo TEM
• Diffraction

Location- The JEOL 200CX system is located in the Building 1 Basement lab of the IRF.

 

JEOL 6300V Scanning Electron Microscope

Description- The JEOL 6300V scanning electron microscope is a fully integrated digital high vacuum system. The system in the IRF is equipped with:

• Tungsten filiment
• High resolution secondary and TV rate backscatter detectors
• Resolution in secondary mode of 3.5nm
• Resolution in backscattered mode of 10nm
• Sample size of up to 5 inches in diameter can be fully imaged
• Motorized X-Y stage that can be driven to stored positions
• Preset Z working distances from 8, 15, 25, 39, and 48mm
• Eucentric stage with tilt capablilty of -5 to + 90 at a working distance of 48mm
• 360 rotation
• Accelerating voltages from 0.5-30kV
• Magnifications from 5X-300,000X
• Visions Software Package
• Polaroid film and digital image storage

Applications-

• Imaging surface morphology of biological and materials samples
• Imaging cell:cell and cell:ECM interactions
• Colloidal gold immunocytochemistry of surface molecules

Location- The JEOL 6300V system is located in the Building 1 Basement lab of the IRF.

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Equipment for Specimen Processing:

The IRF is equipped with a wide range of specimen processing equipment for both scanning and transmission electron microscopy including the necessary centrifuges, vacuum ovens, Tousimis Samdri-PVT-3B critical point dryer, Polaron sputter coater and Leica Ultracut R ultramicrotomes.

 

BioRad SEM Coating System

Tousimis Samdri-PVT-3B Critical Point Dryer

Leica Ultracut R