Histology and Light Microscopy

Equipment for Specimen Processing- A fully equipped histology facility for individual or service processing and sectioning of tissues for light microscopy is available in Building 1 Room B60 of the IRF. Available equipment includes:

• Citadel 1000 Tissue Processor capable of automated computer controlled processing of up to 50 standard tissue cassettes simultaneously

• PELCO Biowave microwave processing center with vacuum infiltration and Coldspot attachments for even distribution of microwave heating

• Triangle Biomedical Sciences Embedding Station with side-by-side platforms for embedding and cooling paraffin blocks

• Two Reichert-Jung 2030 rotary microtomes

• Zeiss Microm 505HN Cryostat

• Vibratome Tissue Slicers

  Applications- Equipment and protocols exist within the IRF for virtually all histological applications from routine hematoxylin and eosin staining through more detailed techniques such as immunohistochemistry.

Imaging Systems for Light Microscopy- Several microscopes are available for routine brightfield, darkfield and fluorescence imaging.

Nikon E600 Widefield Epifluorescence and Darkfield Microscopy System

Description- The Nikon E600 configuration consists of:

• Excitation and emission filters for UV, green, red and far red dyes
• Fiber optic darkfield illuminator for high-resolution darkfield imaging
• Wide range of Plan and Plan-Fluor objectives ranging from 4X through 100X oil
• Phase and DIC objectives and condensers
• Micropublisher digital camera with Qimaging software

Location- The E600 system is located in the Building 1 Basement lab of the IRF

 

Nikon Optiphot Epifluorescence System

Description- The Nikon Optiphot Epifluorescence system configuration consists of:

• Excitation and emission filters for red/green wide field fluorescence
• 4,10,40 and 60X oil objectives
• Zeiss Axiocam MRc digital camera and Axiovision software

Location- The Optiphot system is located in Building 1 Room B60.

 

Zeiss SteREO Lumar V.12 Stereomicroscope

Description- The Zeiss Lumar V.12 system consists of:

• Filter sets for fluorescent imaging of EGFP, Rhodamine, CFP, YFP, Cy3 and DAPI ranges of dyes
• LCD cold light source for transmitted light imaging
• Fiber optic illumination source
• Zeiss Axiocam MRm digital camera with Axiovision software

Location- The Lumar V.12 system is located in Building 1 Room B60.

Website- www.zeiss.com

 

Nikon SMZ 1500 Stereomicroscope

Description- The Nikon SMZ 1500 Stereomicroscope system consists of:

• Filter sets for fluorescent imaging of EGFP and red ranges of dyes
• Transmitted light imaging

Location- The Nikon SMZ 1500 system is located in the Building 1 Basement lab.