Whole Animal Imaging

Visual Sonics VEVO 660 Ultrasound

Description - The VEVO 660 is an in vivo microimaging system designed for non-invasive small animal research. The system offers spatial resolution down to 30mm and allows real time longitudinal studies of mice and rats from embryonic day 5.5 (mouse) through to the adult. The IRF system is equipped with:

• Both mouse and rat platforms designed to maintain body temperature during anesthesia
• Compact isoflurane anesthesiology system with an oxygen regulator and flow meter

Software packages:

• Analytical software for B-mode image capture and analysis, ECG on screen tracing, measurements and annotations, cineloop image review capture and display
• M-Mode capture and analysis software for characterization of vessel and heart dynamics including wall thickness
• Pulsed Wave Doppler package enabling quantitative analysis of blood flow velocity and waveform measurements

3 Scanheads:

• 37.5MHz/Focal length of 15mm/Field of view of 20.8mm – Rat cardiology, EKV, General Imaging
• 50MHz/ Focal length of 12.7mm/Field of view of 20mm – Adult mouse cardiology
  20mm – Adult mouse cardiology
• 82.5MHz/Focal length of 4.5mm/Field of view of 17.4mm – Surface imaging (epidermis, skin tumors, bowel imaging, peritoneum)
  

Applications:

• Cardiovascular- enables real time imaging and analysis of physiological function of the heart chambers and associated major vessels and the ability to monitor morphological changes during heart development and pathology.
• Cancer biology- enables 2D and 3D tracking of the shape and size of subcutaneous and orthotopic tumor growth in longitudinal studies of the same animal during therapeutic treatment. Software allows volumetric analysis of tumor size and tracking of tumor vasculature and blood flow.
• Neurobiology- enables real time monitoring of embryonic or neonatal brain development prior to ossification of the skull, cell lineage studies, identification of progressive neural degenerative diseases, and visualization of image guided needle injections and extractions.
• Developmental biology- longitudinal and dimensional assessment of the same animal from early in development through adulthood, analysis of timed pregnant animals for developmental stage.

Location- The VEVO 660 is located in Building 1 Room B51.

Website- www.visualsonics.com

 

JL Shepherd and Associates Model 143-68 Blood Product Irradiator

Description- The Model 143 Cesium-137 gamma irradiator is a self-contained fully shielded unit capable of delivering a dose rate of up to 200 Roentgen/minute. Final sample dose is determined by duration of irradiation that can be controlled by a preset timer on the unit.

Applications- Irradiation of molecules, cells, tissues, organs or whole animals.

Prior to use all researchers must complete a radiation saftey course. After completion of the radiation safety course individuals should contact Dr. Robert Price to set up a training time and to obtain security clearance via a palm reader for access to the unit.

 

Kodak Image Station 2000MM

Description- The Kodak ISM 2000MM is a fluorescence based molecular imaging station equipped with long wavelength full field illumination. Depending on available filters placed in the system green to near IR excitation and emission can be used for imaging luminescent, fluorescent, radiographic and colorimetric labeled biomolecules in a variety of formats including membranes, electrophoresis gels, plates and tissues.

• Available excitation filters are for wavelengths of 465nm, 535nm and 620nm. Corresponding emission filters are for 533nm, 600nm and 700nm.
• Kodak 1 D Image Analysis Software

Applications- Documentation and analysis of:

• DNA, RNA and protein electrophoresis gels
• Southern, Northern and Western blots
• Dot and Slot blots
• Microplate assays
• Macro Arrays
• Automated cell colony counting
• Plaque screening
• Thin layer chromatography

Location- The ISM2000MM is located in Building 1 Room B51.

Website- www.jaewootr.com/kodak/ISM2000MM/is2000mm.html